Introduction and Workflow
- Amplicon sequencing focuses on the targeted amplification and sequencing of specific DNA regions enabling researchers to analyze genetic variation in specific genomic regions.
- The workflow begins with DNA isolation, followed by PCR amplification of target regions. Barcoded strand-specific libraries are then prepared using adapters, allowing different samples to be pooled together.
- This multiplexing enables the simultaneous targeting of hundreds of genes from various libraries in a single run.
- This method is particularly useful for studying genetic variation, microbial communities, and evolutionary relationships. By selectively amplifying regions of interest, such as 16S rRNA for bacteria or ITS regions for fungi.
Advantage
- Unlike targeted or exome sequencing, amplicon sequencing narrows down to even smaller, highly specific regions, resulting in a more efficient and focused approach for certain applications, such as microbial diversity studies or detecting rare variants.
- Capable of detecting low-abundance species or genetic variants, providing specific resolution of target regions.
- Focuses on specific genomic regions, allowing for detailed and precise analysis.
- Provides quick insights into genetic composition and diversity, facilitating timely research and diagnostics.
Applications of Amplicon Sequencing
- Microbial Diversity and Ecology- Offers comprehensive profiles of microbial communities by focusing on specific genes, such as the 16S rRNA gene in bacteria, to analyze microbial diversity, community structure, and interactions within various environments.
- Pathogen Detection and Identification- Provides rapid and precise identification of pathogens, including bacteria, viruses, and fungi, through the amplification and sequencing of targeted genomic regions linked to disease-causing organisms.
- Genetic Variation and Mutation Analysis- Targets specific genomic regions to detect genetic variations, mutations, or polymorphisms associated with diseases or traits, enabling focused genetic research and advancements in personalized medicine.
- Cancer Research and Diagnostics- Helps identify cancer-related mutations and genetic alterations by sequencing targeted genes or exons within tumor samples, enhancing our understanding of cancer biology and supporting the development of targeted treatments.
- Environmental Monitoring- Applies amplicon sequencing to evaluate the effects of environmental changes on microbial communities, track specific species, and assess ecosystem health and biodiversity.
- Plant Genomics- Explores genetic markers associated with important agronomic traits, aiding in crop improvement and breeding programs.
- Genome-Wide Association Studies (GWAS)- Links genetic variations to specific traits, advancing selective breeding and improving understanding of genetic disorders in livestock.
Service Specifications
Sample Requirement
Genomic DNA, Cultivated cells, Blood, tissues, FFPE (formalin-fixed paraffin-embedded) and Other samples.
Please refer to sample submission guidelines or Contact Us!
Sequencing Platform
Illumina NovaSeq 6000/ NovaSeq X
Deliverables
- The original sequencing data
- Experimental results
- Bioinformatics and Data Analysis Report
- Details of Amplicon Sequencing (customizable)