Degradome Sequencing

Introduction and Workflow

Degradome sequencing, also called Parallel Analysis of RNA Ends (PARE), is a high-throughput technique to analyze RNA degradation products, uncovering RNA stability, miRNA targets, and regulatory networks.
Degradome Sequencing Workflow combines next-generation sequencing (NGS) with a modified 5' RACE method, involving RNA extraction, adapter ligation to capture degradation products, cDNA synthesis, and high-throughput sequencing to map RNA cleavage sites.
Bioinformatics analysis for Degradome Sequencing include data preprocessing, aligning reads to reference genomes, identifying miRNA/ta-siRNA cleavage sites, and quantifying cleaved targets, revealing RNA decay dynamics and regulatory mechanisms.
The method facilitates the discovery of novel miRNAs and ta-siRNAs, studies on miRNA-mediated gene regulation, and comparative RNA decay analysis across species, contributing to a comprehensive understanding of RNA life cycles.

Degradome sequencing provides a focused analysis of RNA degradation, specifically targeting cleavage sites mediated by miRNAs and ta-siRNAs, making it ideal for studying gene regulation and RNA stability.
By targeting degradation products, degradome sequencing minimizes the impact of intact RNAs, enhancing sensitivity for detecting miRNA targets and cleavage events.
Degradome sequencing focuses on RNA cleavage sites, eliminating the need for additional steps like ribosomal RNA removal, making it a more cost-effective alternative to broader transcriptome studies.
Degradome sequencing enables the identification of novel regulatory elements, such as circRNAs and biomarkers, expanding its utility in understanding RNA regulatory networks.

Gene Regulation Studies- Used to identify miRNA targets and cleavage sites, elucidating the regulatory networks that control gene expression and RNA turnover in different organisms.
Developmental Biology- Explores RNA degradation patterns during developmental stages, uncovering the molecular mechanisms underlying cell differentiation and tissue formation.
Disease Research- Investigates RNA cleavage profiles associated with diseases such as cancer and neurodegenerative disorders, helping to identify potential biomarkers and therapeutic targets.
Functional Genomics- Provides a detailed view of RNA decay dynamics, enabling the study of gene function and the roles of regulatory RNAs in cellular processes.
Comparative Genomics- Analyzes RNA degradation patterns across species or environmental conditions to understand evolutionary conservation and differences in regulatory mechanisms.
Stress and Environmental Responses- Examines changes in RNA stability under stress conditions, shedding light on the molecular mechanisms of stress adaptation and survival strategies.

Sample type: Total RNA without degradation or DNA contamination

Total RNA≥ 20 μg, Minimum Quantity: 15 μg, Concentration≥ 100 ng/µL

Please refer to sample submission guidelines or Contact Us!

Illumina NovaSeq 6000/ NovaSeq X