Sample Submission Guideline
We humbly offer a wide range of services, including genomics, transcriptomics, metagenomics, epigenomics, single-cell sequencing, genotyping, microarray, bioinformatics, and more. To help us deliver the best results for you, we request you to review our sample requirements and follow the instructions for packaging, labeling, and shipping your samples.
General Guidelines
- Please complete the Sample Initiation Form (SIF), ensuring that the sample names on the form match the labels on the sample tubes. We also request that you send an electronic copy of the form and any required QC data via email.
- Each tube should be labeled on the lid with a maximum of 4-6 alphanumeric characters (e.g., 4B0001). Use a black permanent marker to write sample names on the top and side of each tube. Avoid writing directly on the tube wall or cover with an oil pen.
- DNA can be submitted in DNase-free water, Elution Buffer, or 10mM Tris pH 8.0. DNA samples should have an OD260/280 ratio as close to 1.8~2.0 as possible. All DNA should be RNase-treated and free from degradation or contamination. Ship with ice packs. The total amount of DNA required depends on the specific application.
- RNA can be submitted in RNase-free water, RNA Stabilization Reagent, or 10mM Tris pH 8.0. All total RNA samples should be DNA-free, with an OD A260/A280 ratio ≥ 1.8, A260/230 ratio ≥ 1.8, and a RIN ≥ 6. Ship with dry ice. The total amount of RNA required depends on the specific application. For Long Read Sequencing, RNA samples should have a RIN ≥ 8.
- The listed concentrations should be determined by fluorometry (e.g., PicoGreen/Qubit/RiboGreen). If using spectrophotometry (e.g., Nanodrop), increase concentrations by approximately twofold.
- The quality inspection method for the sizes and concentrations of the Ready To Run Library is Qubit and Agilent Bioanalyzer.