Hybrid Genome Sequencing

Introduction and Workflow

Hybrid sequencing integrates the accuracy of short-read sequencing (Illumina) with the comprehensive coverage of long-read sequencing (PacBio or Nanopore), ensuring detailed and complete genome assemblies.
This approach involves sequencing the same sample with both technologies, which means different libraries are prepared for both the technology followed by sequencing.
Following sequencing, advanced bioinformatics tools are used to align and integrate the long and short reads, enhancing variant detection and improving the resolution of complex genomic regions.
Hybrid sequencing is applicable to a wide range of research areas, from complex genome assemblies to resolving repetitive regions and improving the quality of reference genomes.

Combines long-read and short-read sequencing for more complete and accurate genome assemblies, effectively resolving complex regions and reducing gaps.
Balances the cost efficiency of short-read sequencing with the detailed resolution of long-read sequencing, making it a budget-friendly choice for comprehensive genome analysis.
Enhances detection of structural variants, such as large insertions and deletions, improving the understanding of genetic diversity and disease mechanisms.
Provides better coverage of challenging genomic features, like high GC content and repetitive sequences, by leveraging the strengths of both sequencing technologies.

Comprehensive Genome Mapping- Hybrid genome sequencing is used to create detailed genome maps by combining the strengths of both short-read and long-read technologies, leading to more complete and accurate assemblies of complex genomes.
Structural Variant Detection- This approach is effective in identifying structural variants, such as large deletions, duplications, and insertions, which are often challenging to detect with short-read sequencing alone.
Genetic Diversity and Evolutionary Studies- It facilitates the study of genetic diversity and evolutionary processes by providing high-resolution data on genomic variations across different species and populations.

Blood, Tissue, and other samples

Please refer to sample submission guidelines or Contact Us!

Illumina NovaSeq 6000/ NovaSeq X and PacBio/Oxford Nanopore